Journal: Fundamental Research
Article Title: Harmine ameliorates steroid-induced osteonecrosis of the femoral head by modulating metabolism and ferroptosis via HIF1-α
doi: 10.1016/j.fmre.2023.08.015
Figure Lengend Snippet: HIF1-α maintained the NADPH/NADP+ and GSH/GSSG ratios to maintain redox balance. (a) KEGG pathway annotation analysis of the 1097 DEPs in proteomics. (b) KEGG pathway annotation analysis of the 44 genes in the intersection result between DEPs (1097) in proteomics and DEGs (758) in transcriptomics. (c) Graphical abstract for subsequent metabolite detection assays. (d) Glucose Uptake Probe-Green was used to detect the glucose uptake ability of BMSCs. (e) Detection of the NADPH/NADP+ ratio in BMSCs. (f) Detection of the GSH/GSSG ratio in BMSCs. (g) Detection of total GSH in BMSCs. (All bar graphs are the mean ± SD; * p < 0.05 and ** p < 0.01 compared with the 100 µM MP treatment group; ns: no significant difference; # p < 0.05 and ## p < 0.01; n = 3 per group).
Article Snippet: Glucose uptake assays were performed using Glucose Uptake Probe-Green (DOJINDO Laboratories, Japanese: #UP02).
Techniques: Transcriptomics